Somaclonal variation in banana (Musa acuminata cv. ''Grande Naine'). Genetic mechanism, frequency, and application as a tool for clonal selection

• Chapter Authors : Khayat, E.; Duvdevani, A.M.; Lahav, E.; Ballesteros, B.A.


• Document type : Conference paper


• Year of publication : 2004


• Book title : Banana improvement: cellular, molecular biology, and induced mutations


• Editors : Jain, S.M.; Swennen, R.


• Publisher(s) : Science Publishers


• Place of publication : Enfield (USA)


• Pages : 97-109


• Language(s) : English


• Abstract : Clonal selection of banana (Musa acuminata AAA, cv. 'Grande Naine') was performed under the marginal climatic conditions for banana cultivation in the Western Galilee region of Israel. The initial selection was carried out on 300 mats originating from six mother clones multiplied by meristem culture. Five clones were selected for further experiments. In the third growth cycle the total number of bunches per hectare of three out of the five selections, exceeded that of the control, while the remaining two selections performed similar or below the control. In the fourth cycle all the selections outperformed the control. Average bunch weights of two of the selected clones were significantly higher than the control. The total yield, derived from bunch weight and the number of bunches per mat of each of the selections, was higher tan that of the control in both the third and the fourth cycle. Hundred and fifty hectares of micropropagated plants derived from the selected clones were planted in the Philippines, and their performance was evaluated and compared to that of a local selection of 'Grande Naine'. The yield of the Israeli selected clones in the Philippines was significantly higher by an average value of 18 percent compared to the local clones. Using degenerate primers, a fragment of a retro-transposable element (BR-1) that was induced by extensive in vitro culture was detected in Musa. Transcriptional activation of the banana retro-transposon was detectable by RT-PCR in the 'Long and Narrow Leaf' mutant (LNL). The frequency of occurrence of BR- 1 was analyzed in genomic DNA of the LNL mutant and in his originator non-mutant mother plant. The analysis revealed amplification of BR-1 in the mutant compared to the mother stock DNA. We postulate that retro-transposable elements are at least in part responsible for the occurrence of the LNL mutant. (Author's abstract).


• Keywords : INDUCED MUTATION; SELECTION; PCR; SOMACLONAL VARIATION; GRANDE NAINE; FIELD TRIALS; PHILIPPINES; ISRAEL


• Open access : Yes


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN040374

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