Add to my selection | 1578 views
Transgenic banana plants expressing a Stellaria media defensin gene (Sm-AMP-D1) demonstrate improved resistance to Fusarium oxysporum
- Authors : Ghag, S.B.; Singh Shekhawat, U.K.; Ganapathi, T.R.
- Document type : Journal article
- Year of publication : 2014
- Journal title : Plant Cell, Tissue and Organ Culture (PCTOC)
- Volume (number) : 119 (2)
- Pages : 247-255
- Peer-reviewed : Yes
- ISSN : 1573-5044
- Language(s) : English
- Abstract : Plant defensins are group of small, cysteine stabilized antimicrobial peptides rich in basic amino acids which inhibit growth of a multitude of phytopathogens. These defensins have been explored widely to generate transgenic crop plants resistant to varied fungal and bacterial diseases. In the present study, gene sequence coding for a seed defensin (Sm-AMP-D1) of common chickweed Stellaria media was synthesized artificially and cloned downstream of a strong constitutive promoter in pCAMBIA-1301 plant expression vector. Transgenic banana plants expressing the Sm-AMP-D1 gene were subsequently generated via Agrobacterium-mediated genetic transformation. Transgenic nature of the regenerated banana plants was confirmed by genomic DNA PCR and Southern blotting analysis. Northern blots demonstrated efficient expression of Sm-AMP-D1 mRNA in transgenic banana plants. Further, two selected transgenic lines challenged with a pathogenic isolate of Fusarium oxysporum f. sp. cubense race 1 showed improved resistance as compared to untransformed control banana plants. These transgenic lines continued to show resistance against Foc race 1 6 months post-infection. This study demonstrates that overexpression of potent plant defensins such as Sm-AMP-D1 in important food crops like banana can lead to development of durable resistance against fungal pathogens.
- Keywords :
DISEASE RESISTANCE;
FUSARIUM OXYSPORUM F. SP. CUBENSE;
RNA;
GM BANANA;
DEFENCE MECHANISMS;
GENOMICS
- Open access : No
- Document on publisher's site : View article on publisher's site
- Musalit document ID : IN140370
If you want to link this record, use this url : www.musalit.org/seeMore.php?id=15293
Generate Citation