A reliable IC one-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm

  • Authors : Iskra-Caruana, M.L.; Galzi, S.; Laboureau, N.

  • Document type : Journal article

  • Year of publication : 2008

  • Journal title : Journal of Virological Methods

  • Volume (number) : 153 (2)

  • Pages : 223-231

  • Peer-reviewed : Yes

  • ISSN : 0166-0934

  • Language(s) : English

  • Abstract : An immunocapture (IC) One-step RT-PCR assay was developed to improve the detection of Banana bract mosaic virus (BBrMV) in single and bulked samples of banana plants. In this paper, an atypical strain of BBrMV was described, the BBrMV "Ref" strain, and we showed that detection with available BBrMV tools using ELISA and RT-PCR approaches was not reliable. Primer sets Bract N1/NR and N2/NR specific to BBrMV were designed and used in RT-PCR and IC-RT-PCR assays with two commercial kits that allow the RT and the PCR reactions to take place simultaneously in the same tube. The new assay enabled detection of BBrMV in leaf extract diluted up to 1Î10?10 and in bulked samples of 10 plants, and was proposed as a new international standard to index BBrMV. (Author's abstract).


  • Open access : No

  • Document on publisher's site : close View article on publisher's site

  • Musalit document ID : IN090044

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